While the Delvotest® range of products is validated primarily for use with raw milk, there are instances where testing other dairy matrices—such as milk powders, creams, and whey—may be necessary. These products differ from raw milk in various physical and chemical properties, which can affect the performance of microbial inhibition tests like Delvotest® T with the Delvotest® Accelerator Smart.
Complication
Testing other dairy matrices presents several challenges:
- Absence of Defined Maximum Residue Limits (MRLs)
Unlike raw milk, processed milk products lack established MRLs due to variability in processing methods and product concentrations. This makes it difficult to standardize testing thresholds for antibiotic residues in these products.
- Variability in Physical and Chemical Properties
Other dairy matrices may have different concentrations, solids content, or pH levels compared to raw liquid milk. For example, milk powders (after reconstitution) can be more concentrated, and alkaline whey has a higher pH. These differences can influence the performance.
Resolution
To address these challenges and ensure accurate testing, follow these steps:
- Follow IDF guidelines for sample preparation
Document FIL-IDF B-471 Detection of Inhibitors and Antimicrobial Residues in Milk and Dairy Products by Screening Methods—Guidance on Preparation of the Test Portion provides detailed instructions on how to prepare different dairy matrices for testing, ensuring compatibility with microbial inhibition tests. It can be accessed via the following links:
https://www.fil-idf.org/wp-content/uploads/woocommerce_uploads/2014/03/B471-2014Inhibitors-and-antimicrobial-residues-in-milk-and-milk-products.CAT_-rts6zg.pdf
https://shop.fil-idf.org/collections/publications/products/detection-of-inhibitors-and-antimicrobial-residues-in-milk-and-dairy-products-by-screening-methods-guidance-on-preparation-of-the-test-portion
Before testing any unknown samples, it is essential to validate your Delvotest® T plates using the specific dairy matrix you intend to analyse. Begin by preparing known negative samples of the dairy matrix to determine the appropriate incubation time, accounting for any matrix-specific factors that may influence the test's timing. Next, prepare positive control samples by spiking the dairy matrix with known concentrations of antibiotics relevant to that product. This step confirms the test's sensitivity and ensures it can reliably detect antibiotic residues in the specific matrix. By thoroughly validating with both negative and positive controls, you establish baseline performance metrics tailored to the dairy product you're testing, ensuring accurate and reliable results.
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