The plate has 8 rows (A-H) and 12 columns (01-12) and each cup has its own ID, for example E06. For each cup the color is analyzed, and the results are presented per cup, denoted by its ID. A plate is filled with different milk samples and it can be desired to assign additional information to each cup, linking it to the milk sample used. This information for the entire plate is called “layout definition”. The layout definition defines for a cup, the following:
• If the cup is a positive reference (Boolean value).
• If the cup is a negative reference (Boolean value).
• Additional information or description of the cup content (string).
Depending on the way-of-working, the layout definition can be provided in a different manner. The following ways of working have been considered:
1. No additional information is required, or no layout-definition has been defined
The fields “Default layout definition” and “Use predefined layout” from the “Setup” page should be empty. No information inserted for the wells.
2. Plates are always filled in the same manner and always the same layout-definition should be used.
On forehand a layout-definition should be created (see How to create a (default) Layout definition) and the name of the layout-definition should be filled in the field “Default layout definition” from the “Setup” page. The field “Use predefined layout” from the “Setup” page should be empty. The information inserted for each well will be used for all the incubations.
3. Plates are filled in ad-hoc manner (Information for each well is requested)
The additional information of each cup can be inserted via the browser during the filling process (Figure below). Click on "Positive" to label a known positive sample as a positive milk control and "Negative" box for a known negative milk control.
Deselect any unused strips if applicable. See also "How do I start a new test in the DAS"
The box “Use predefined layout” from the “Setup” page should be empty.
4. Several different standard plate filling layouts are used (Load choice)
For each plate filling layout, a layout definition should be created . Before the incubation starts, the operator should select and load (incubation page) the applicable layout-definition. The fields “Default layout definition” and “Use predefined layout” from the “Setup” page must be empty.
NOTE: the Delvotest plate will be 180° rotated in the DAS heating grid. A01 well is located on the lower right corner of the grid.
5. The layout of the plate is known on forehand
The layout-definition can be prepared on forehand and must be defined in a csv-file. The creation of the csv-file is described here. The filename (e.g. 18C27B30-216.csv) of the prepared csv-file should be equal to the unique ID (e.g. 18C27B30-216) of the plate, which contains of the batch-id (e.g. 18C27B30) and the plate number (e.g. 216, no leading 0). The batch-id and plate number can be found on the barcode sticker on the plate. When the barcode is read by the DAS, it can load automatically the prepared layout-definition (predefined layout). This automatic loading of the unique (predefined) layout-definition can be enabled by checking the checkbox “Use predefined layout” from the “Incubation” or “Setup” page (will remain as standard setting).